M4892
McCoy′s 5A Medium
Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Fabrication pharma
Synonyme(s) :
5A basal medium
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About This Item
Produits recommandés
Niveau de qualité
Forme
powder
Technique(s)
cell culture | mammalian: suitable
Composants
phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no
Conditions d'expédition
ambient
Température de stockage
2-8°C
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Description générale
McCoy′s 5A Medium is recommended to produce viruses in primary cell cultures.
Application
McCoy′s 5A Medium has been used for culturing:
- human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b, and TENN) to study the function of leucine-rich repeat-containing G-protein-coupled receptor (LGR5) in colon cancer metastasis
- human colorectal HCT116 cells to study miR-224 over-expression in cancer
- osteoblasts to study ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media
Actions biochimiques/physiologiques
McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.
Quantité
Formulated to contain 11.9 grams of powder per liter of medium.
Reconstitution
Supplement with 2.2 g/L sodium bicarbonate.
Souvent commandé avec ce produit
Réf. du produit
Description
Tarif
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
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Les clients ont également consulté
Yu Wang et al.
PloS one, 8(7), e68744-e68744 (2013-08-08)
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