M4812

Anti-Myosin Va (LF-18) antibody produced in rabbit

~0.4 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-GS1, Anti-MYH12, Anti-MYO5, Anti-MYR12
• Numéro MDL: MFCD03455613
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 190 kDa
• Espèces réactives: chicken, rat
• Validation améliorée: independent; Learn more about Antibody Enhanced Validation
• Concentration: ~0.4 mg/mL
• Technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using rat and chicken cerebellum sections; microarray: suitable; western blot: 1:1,000 using a rat brain extract
• Numéro d'accès UniProt: Q02440
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... MYO5A(4644) ; mouse ... Myo5a(17918) ; rat ... Myo5a(25017)

Description

Description générale

Anti-myosin Va (LF-18) recognizes myosin Va (190kDa). Myosin 5a is encoded by the gene mapped to human chromosome 15q21.2. The encoded protein is a member of the unconventional myosin family. It is a two-headed motor containing two identical heavy chains and multiple light chains. Myosin Va is widely expressed, but at high levels in the brain, testes and melanocytes.

Immunogène

synthetic peptide located near the C-terminus of chicken myosin Va (amino acids 1782-1799 with N-terminally added lysine) conjugated to KLH. This sequence is identical in human, mouse and rat.

Application

Anti-Myosin Va (LF-18) antibody produced in rabbit has been used in:

• Western blot.
• In situ proximity ligation assay (PLA).
• Glutathione S-transferase (GST)-spinophilin co-sedimentation assay.

Actions biochimiques/physiologiques

Myosin 5a is an actin-based molecular motor responsible for transport of secretory granules in various cell systems, including insulin granule. Additionally, it also mediates the transport of cargo such as cell-surface receptors, pigment and organelles like endoplasmic reticulum to the plasma membrane. The encoded protein facilitates pigmentation and synaptic activity in the central nervous system. Genetic variations in the gene are associated with the development of immunological and neurological diseases, including Griscelli syndrome type 1 and Elejalde syndrome.

Forme physique

Solution in 0.01 M phosphate buffered saline containing 1% BSA and 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2-8°C for up to one month. For prolonged storage, freeze in working aliquots at -20°C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Autres remarques

In order to obtain best results in different techniques and preparations we recommend determining optimal working dilutions by titration test.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable