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L9381

Sigma-Aldrich

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

FITC conjugate, lyophilized powder

Synonyme(s) :

Bandeirea simplicifolia agglutinin, BS-I

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

Bandieraea simplicifolia

Niveau de qualité

200

Conjugué

FITC conjugate

Forme

lyophilized powder

Puissance

<100 μg per mL agglutination activity (using human blood group A erythrocytes)

Composition

Protein, ~10% Lowry

Ampleur du marquage

1-3 mol FITC per mol protein

Température de stockage

−20°C

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Actions biochimiques/physiologiques

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Autres remarques

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Forme physique

Contains phosphate buffer salts and sodium chloride

Remarque sur l'analyse

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Jing Feng et al.
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The Journal of biological chemistry, 295(28), 9618-9629 (2020-05-24)
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American journal of respiratory and critical care medicine, 196(8), 1075-1077 (2017-03-10)
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The maintenance of endothelial cell-cell junctions is vital for the control of blood vessel leakage and is known to be important in the growth and maturation of new blood vessels during angiogenesis. Here we have investigated the role of a
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