L9152

Anti-Leukemia Inhibitory Factor antibody produced in goat

IgG fraction of antiserum, lyophilized powder

• Synonyme(s) : CDF, D factor, DIA, Emfilermin, LIF, differentiation inhibitory activity
• Numéro MDL: MFCD00283185
• Code UNSPSC : 51111800
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: goat
• Niveau de qualité: 100
• Conjugué: unconjugated
• Forme d'anticorps: IgG fraction of antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: lyophilized powder
• Espèces réactives: mouse
• Technique(s): immunohistochemistry: suitable; neutralization: suitable; western blot: suitable
• Numéro d'accès UniProt: P09056
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... LIF(3976) ; mouse ... Lif(16878)

Description

Spécificité

The antibody neutralizes the biological activity of recombinant mouse LIF and also neutralizes the biologicl activity of recombinant human LIF at a 10-fold higher IgG concentration. Based on ELISA and immunoblotting, this antibody shows <10% cross-reactiity with recombinant human LIF.

Immunogène

purified, E. coli-derived, recombinant mouse LIF.

Application

For Western blot a concentration of 0.1 μg/ml is recommended. For immunohistochemistry, a working dilution of 5-15 μg/ml is recommended. The neutralization dose is typically 80-480 ng/mL in the presence of 3 ng/mL Recombinant Mouse LIF.

Optimal dilutions should be determined by the end user.

Actions biochimiques/physiologiques

Leukemia inhibitory factor (LIF) was initially identified as a factor that inhibited the proliferation and induced the differentiation to macrophages of the murine myeloid leukemic cell line M1. Subsequent to its purification and molecular cloning, LIF was recognized to be a pleiotropic factor with multiple effects on both hematopoietic and non-hematopoietic cells. LIF has overlapping biological functions with OSM, IL-6, IL-11 and CNTF. All these cytokines utilize gp130 as a component in their signal transducing receptor complexes. Mouse LIF cDNA encodes a 203 amino acid residue polypeptide with a 23 amino acid signal peptide that is cleaved to yield a 180 amino acid mature mouse LIF. Native human and mouse LIF are highly glycosylated monomeric proteins. Both human and murine LIF protein sequences have multiple potential N- and O-linked glycosylation sites and six conserved cysteine residues that are involved in three intramolecular disulfide bridges.

Forme physique

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose.

Notes préparatoires

Protein A or G purified

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)