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Key Documents

L8507

Sigma-Aldrich

Luciferase from Vibrio fischeri (Photobacterium f)

lyophilized powder

Synonyme(s) :

Bacterial Luciferase, Luciferase from Photobacterium fischeri

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About This Item

Numéro CAS:
Numéro de classification (Commission des enzymes):
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Forme

lyophilized powder

Niveau de qualité

Composition

Protein, ~40% biuret

Température de stockage

−20°C

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Application

Luciferase from Vibrio fischeri has been used in a study to assess kinetics of light emission and oxygen consumption by bioluminescent bacteria. It has also been used in a study to investigate the sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species.

Caractéristiques et avantages

Partially purified, soluble extracts containing FMN-dependent luciferase and NADH- and NADPH-dependent FMN reductases. Produces light in a system containing FMN, NADH or NADPH, and n-decyl aldehyde.

Autres remarques

ATCC No. 7744 balance primarily buffer salts and stabilizer.

Forme physique

Partially purified lyophilized powder

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Conseils de prudence

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Les clients ont également consulté

Thomas E Crowley
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology, 39(2), 126-132 (2011-03-30)
The genes responsible for luminescence in various species of the marine microorganism Photobacterium, have been used for many years as a tool by researchers and instructors. In particular, the lux operon of Photobacterium fischeri has been used by many instructors
Loredana Peca et al.
FEMS microbiology letters, 289(2), 258-264 (2008-11-20)
Two whole-cell bioluminescent reporters were constructed by fusing the reporter genes luxAB with the Co(2+) and Zn(2+) inducible coaT promoter or the Ni(2+)-inducible nrsBACD promoter, respectively, in the genome of Synechocystis sp. PCC 6803. The obtained reporters, designated coaLux and
Prashant K Sharma et al.
Surgery, 147(1), 89-97 (2009-09-08)
Bacterial peritonitis is a life-threatening abdominal infection associated with high morbidity and mortality. The rat is a popular animal model for studying peritonitis and its treatment, but longitudinal monitoring of the progression of peritonitis in live animals has been impossible
Xin-Xin Huang et al.
Huan jing ke xue= Huanjing kexue, 29(11), 3159-3165 (2009-02-04)
Recombinate luminescence bacteria have the important role in evaluating water toxicity. Two recombinate luminescence bacteria vectors PUCD-uvrA and PUCD-alkA were constructed to investigate the impaired mechanism of pollutant genetic toxicity. The genes of uvrA and alkA were amplified by PCR
Anya Bakhrat et al.
Cell biology and toxicology, 27(3), 227-236 (2011-03-03)
We describe a Saccharomyces cerevisiae bioluminescence assay for UV and arsenate in which bacterial luciferase genes are regulated by the promoter of the yeast gene, UFO1. UFO1 encodes the F-box subunit of the Skp1–Cdc53–F-box protein ubiquitin ligase complex and is

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