KMM-201NV
KOD One™ PCR Master Mix -BLUE-
Ready-to-use 2X hot-start PCR master mix with a modified KOD DNA polymerase optimized for ultra-fast and convenient high-fidelity PCR
Synonyme(s) :
Fast Hot-start PCR, High-fidelity DNA polymerase, KOD One™ PCR Ready Mix
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About This Item
Code UNSPSC :
41106314
Nomenclature NACRES :
NA.55
Produits recommandés
Source biologique
Escherichia coli (carrying the cloned UKOD DNA polymerase gene)
Niveau de qualité
Forme
liquid
Utilisation
sufficient for 25 reactions
sufficient for 500 reactions
Caractéristiques
Fast PCR
High Fidelity PCR
dNTPs included
hotstart
Technique(s)
PCR: suitable
Entrée
crude DNA
Conditions d'expédition
dry ice
Température de stockage
−20°C
Description générale
KOD One™ PCR master Mix -Blue- is a ready-to-use 2 x PCR master mix containing a novel genetically modified KOD DNA polymerase (UKOD) along with a new elongation accelerator, enabling fast PCR with an extension time of 5 sec/ kb for template DNA <10kb. This master mix has greater efficiency and flexibility than conventional PCR enzymes with an ability to amplify from crude specimens such as blood and mouse cell lysates. In addition to crude samples, it can be used on templates containing uracils (dU). The master mix can use primers containing both inosines (dI) and dU for amplification.
This hot start KOD polymerase is antibody-inactivated with two types of antibodies that prevent the 5′→3′ polymerase activity and 3′→5′ exonuclease activity. Upon heat-activation, the 3′→5′ proof-reading ability of the enzyme generates blunt-end PCR products.
This hot start KOD polymerase is antibody-inactivated with two types of antibodies that prevent the 5′→3′ polymerase activity and 3′→5′ exonuclease activity. Upon heat-activation, the 3′→5′ proof-reading ability of the enzyme generates blunt-end PCR products.
Application
- Genomic DNA amplification
- cDNA amplification
- Direct PCR
- Colony PCR
- Amplification of NGS libraries
- Site direct gene mutation
Compatible Sample Types: Serum, Plasma, Cell, Plant, soil extraction, DNA, nail, hair etc.
Sample Volume Needed: about 50ng DNA
Caractéristiques et avantages
- Fast: Amplifies the targets using the following very short conditions:
o 1∼ 10 kb: 5 sec/ kb
o 10 kb∼: 10 sec/ kb
- Ready-to-use: 2X premixed solution of UKOD DNA polymerase, buffer, dNTPs, and MgCl2. KOD One PCR Master Mix Blue includes a loading dye (BPB) to allow direct loading onto agarose gels.
- High Fidelity: Approximately 80-fold higher fidelity than conventional Taq DNA polymerase. This mix can be used for various purposes where this would be an advantage (e.g., in the preparation of long target amplicons for sequencing).
- High Efficiency: Enables high throughput PCR and ultra-fast PCR cycling conditions with an extension time of as quick as 5 sec/kb.
- Crude Sample PCR: Effective for direct PCR amplification of crude samples such as biological samples, food samples, soil extract, etc.
- High flexibility: Amplifies templates containing uracils (dU). Also, amplifies fragments using degenerate primers with inosines (dI) and uracils (dU)
Conditionnement
KMM-201NV - 5X1ML is sufficient for 500 reactions at 20 μL each or for 200 reactions at 50 μL each.
KMM-201NVS - 0.25ML is sufficient for 25 reactions at 20 μL each or for 10 reactions at 50 μL each.
KMM-201NVS - 0.25ML is sufficient for 25 reactions at 20 μL each or for 10 reactions at 50 μL each.
Stockage et stabilité
Stored at -20 °C for a year. 4 °C for a month.
Autres remarques
For R&D use only. Not for drug, household, or other uses.
Informations légales
*Manufactured by Toyobo and distributed by Sigma-Aldrich. Not available in Japan.
KOD One is a trademark of Toyobo Co., Ltd.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Met. Corr. 1
Code de la classe de stockage
8B - Non-combustible corrosive hazardous materials
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Moe Yokoshi et al.
Molecular cell, 78(2), 224-235 (2020-02-29)
Formation of self-associating loop domains is a fundamental organizational feature of metazoan genomes. Here, we employed quantitative live-imaging methods to visualize impacts of higher-order chromosome topology on enhancer-promoter communication in developing Drosophila embryos. Evidence is provided that distal enhancers effectively
Nuria Cortes-Silva et al.
Current biology : CB, 30(4), 561-572 (2020-02-08)
Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex at centromeres. In most eukaryotes, kinetochore assembly is primed by the histone H3 variant CenH3 (also called CENP-A), which physically interacts with components of the inner kinetochore constitutive centromere-associated network
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