Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

H9166

Sigma-Aldrich

Vascular Endothelial Growth Factor 165 human

≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

Synonyme(s) :

Endothelial Growth Factor, Vascular Growth Factor

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

product name

Vascular Endothelial Growth Factor 165 human, VEGF165, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

Source biologique

human

Niveau de qualité

Produit recombinant

expressed in HEK 293 cells

Pureté

≥95% (SDS-PAGE)

Forme

lyophilized powder

Puissance

≤5 ng/mL EC50

Qualité

endotoxin tested

Poids mol.

dimer 45 kDa (glycosylated)

Conditionnement

pkg of 5x10 μg
pkg of 10 μg

Fabricant/nom de marque

HumanZyme

Conditions de stockage

avoid repeated freeze/thaw cycles

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤1 EU/μg

Numéro d'accès UniProt

Température de stockage

−20°C

Informations sur le gène

human ... VEGFA(7422)

Description générale

Vascular endothelial growth factor 121 (VEGF121) belongs to the PDGF(platelet derived growth factor)/VEGF growth factor family. The VEGFA gene is mapped to human chromosome 6p21.1. The encoded protein is a dimeric glycoprotein consisting two monomers antiparallel to each other. There are five types of VEGF ligands identified in mammals, which exists as different splice variants. Vascular endothelial growth factor 165 (VEGF165) belongs to the PDGF(platelet derived growth factor)/VEGF growth factor family and VEGF is secreted by many cell types.HumanKine VEGF165 is expressed in human HEK 293 cells as a glycosylated homodimer with an apparent molecular mass of 45 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Actions biochimiques/physiologiques

VEGF (vascular endothelial growth factor) acts as a potent angiogenic factor and mitogen that stimulates proliferation, migration and formation of endothelial cells. VEGF stimulates permeabilization of blood vessels and is present in some tumors of the nervous system. VEGFA is known to interact with VEGFR co-receptors such as heparan sulphate proteoglycans and neuropilins. Mutation in the VEGFA gene results in defective vascular development. VEGF is induced by hypoxia, oncogene mutations, and cytokines such as (interleukin) IL-1, IL-8, TNF-α (tumor necrosis factor α).

Forme physique

Lyophilized from a 0.2 μm filtered solution of 1X PBS

Remarque sur l'analyse

The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (human umbilical vein endothelial cells).

Informations légales

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Chunming Li et al.
Molecular and cellular biochemistry, 346(1-2), 173-178 (2010-09-30)
Cell adhesion is an important process during morphogenesis, differentiation, and homeostasis in cell biology. The role of vascular endothelial growth factor (VEGF) in cell adhesion of keratinocytes is unclear. In our study, a human keratinocyte cell line, HaCaT cells, which
Genetic variants on chromosome 6p21.1 and 6p22.3
are associated with type 2 diabetes risk: a case?control
study in Han Chinese
Lu F, et al.
Journal of Human Genetics, 57.5 (2012)
VEGF receptor signalling ? in control of vascular function
Olsson AK, et al.
Nature Reviews in Molecular and Cell Biology, 7.5 (2006)
Predominant role of endothelial nitric oxide synthase in vascular endothelial growth factor-induced angiogenesis and vascular permeability
Fukumura D, et al.
Proceedings of the National Academy of Sciences of the USA, 98.5, 2604-2609 (2001)
Transcriptome-wide Landscape of Pre-mRNA
Alternative Splicing Associated with Metastatic Colonization
Lu ZX, et al.
Molecular Cancer Research (2014)

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique