Accéder au contenu
Merck
Toutes les photos(1)

Documents

H3662

Sigma-Aldrich

HEPES sodium salt solution

1M, BioReagent, suitable for cell culture

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Formule empirique (notation de Hill):
C8H17N2NaO4S
Numéro CAS:
75277-39-3
Poids moléculaire :
260.29
Numéro MDL:
MFCD00036463
Code UNSPSC :
12161700
ID de substance PubChem :
24895590
Nomenclature NACRES :
NA.25

Niveau de qualité

200

Stérilité

sterile-filtered (0.2 μm filtered)

Gamme de produits

BioReagent

Forme

solution

Concentration

0.90-1.10 M

Technique(s)

cell culture | mammalian: suitable

Impuretés

Bioburden, tested
 DNase, RNase. Protease, Nickase, free
 endotoxin, tested
<5 ppm Heavy metals (as Pb)

Traces de cations

Fe: <5 ppm

Chaîne SMILES 

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

Clé InChI

RDZTWEVXRGYCFV-UHFFFAOYSA-M

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Catégories apparentées

Description générale

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.

Application

HEPES sodium salt solution is suitable for use in cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Slide 1 of 3

1 of 3

HEPES sodium salt BioPerformance Certified, suitable for cell culture, &#8805;99.0%

Sigma-Aldrich

H3784

HEPES sodium salt

HEPES potassium salt &#8805;99.5% (titration)

Sigma-Aldrich

H0527

HEPES potassium salt

EmbryoMax&#174; 1M HEPES Buffer Solution, Liquid, 1M The EmbryoMax 1M HEPES Buffer Solution, Liquid, 1M is available in a 100 mL format and may be used for routine mouse embryonic stem cell culture applications.

Sigma-Aldrich

TMS-003

EmbryoMax® 1M HEPES Buffer Solution, Liquid, 1M

Hydrogen ion buffers for biological research.
Good NE, et al.
BioChemistry: An Indian Journal, 5, 467-477 (1996)
I J Chopra et al.
Thyroid : official journal of the American Thyroid Association, 6(4), 255-259 (1996-08-01)
We have devised a practical, sensitive, and reliable assay for measurement of free T3 concentration in serum. The assay employs a convenient and disposable plastic equilibrium dialysis cell and a buffer that resembles the in vivo biochemical environment (Nelson JC
C Estrada et al.
The Biochemical journal, 326 ( Pt 2), 369-376 (1997-09-18)
Although it has been demonstrated that NO inhibits the proliferation of different cell types, the mechanisms of its anti-mitotic action are not well understood. In this work we have studied the possible interaction of NO with the epidermal growth factor
S Hugel et al.
British journal of pharmacology, 166(8), 2402-2416 (2012-03-29)
Artificial buffers such as HEPES are extensively used to control extracellular pH (pH(e) ) to investigate the effect of H(+) ions on GABA(A) receptor function. In neurones cultured from spinal cord dorsal horn (DH), dorsal root ganglia (DRG) and cerebellar
Jihye Park et al.
The Analyst, 137(19), 4411-4414 (2012-08-07)
We have developed a quickly responsive, and specific fluorescent assay for the detection of Au(III) on the basis of the formation of gold nanoparticles in the presence of HEPES, which cleave the C-I bond of I-BODIPY 1 to yield the
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique