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GE29-0485-86

HisTrap™ Excel

Cytiva 29-0485-86, pack of 1 mL

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About This Item

Code UNSPSC :
23151816
Nomenclature NACRES :
NA.56

Conditionnement

pack of 1 mL

Fabricant/nom de marque

Cytiva 29-0485-86

Disponibilité

not available in North America

Paramètres

1 ml/min - 4 ml/min flow rate
5 bar

Taille du lit

7 mm × 25 mm

Volume du lit

1 mL

D.I. de la colonne

7 mm

Matrice

highly cross-linked 6% agarose

Diamètre moyen

90 μm

cleaning

2-14

working range

2-13

Description générale

Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.
HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). HisTrap excel 1 mL and 5 mL are ready-to-use IMAC columns prepacked with Ni Sepharose excel. The design of the columns in combination with the specific properties of the resin enables fast and convenient purifications. The special type of filter in the top and bottom of the columns makes it possible to load large volumes of cell-free, unclarified samples directly on the columns without causing back pressure problems. This time-saving property helps prevent degradation and loss of sensitive target proteins.

Caractéristiques et avantages

  • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling
  • HisTrap excel columns allow direct purification of cell-free, unclarified samples
  • HisTrap excel columns are made of biocompatible polypropylene. The columns are delivered with a stopper on the inlet and a snap-off end on the outlet.
  • HisTrap excel columns can be operated either with a peristaltic pump or with chromatography systems such as ÄKTA systems. The columns cannot be opened or refilled.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Autres remarques

H2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 1 ml/min is recommended.

Informations légales

HisTrap is a trademark of Cytiva

Pictogrammes

Environment
GHS09

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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