GE17-0407-03
HiTrap® Heparin High Performance
Cytiva 17-0407-03, pack of 5 × 5 mL
Synonyme(s) :
Heparin HP columns
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About This Item
Code UNSPSC :
23151816
Nomenclature NACRES :
NA.56
Produits recommandés
ligand
porcine heparin
Conditionnement
pack of 5 × 5 mL
Fabricant/nom de marque
Cytiva 17-0407-03
Paramètres
42 psi
Taille du lit
16 mm × 25 mm
Volume du lit
5 mL
D.I. de la colonne
16 mm
Matrice
highly cross-linked 6% agarose
Taille moy. des particules
34 μm
cleaning
5-10
working range
5-10
Description générale
HiTrap® Heparin HP columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification of proteins with affnity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.
Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.
Heparin is coupled to the Sepharose™ High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap® Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap® Heparin HP columns equilibrated with 25 mL of 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily performed with a syringe and the supplied Luer adapter. Seal the column with the supplied stopper and domed nut. The recommended storage temperature is 4° C to 30° C.
Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.
Heparin is coupled to the Sepharose™ High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap® Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap® Heparin HP columns equilibrated with 25 mL of 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily performed with a syringe and the supplied Luer adapter. Seal the column with the supplied stopper and domed nut. The recommended storage temperature is 4° C to 30° C.
Caractéristiques et avantages
- Heparin binds to a wide range of biomolecules via both affinity and ion exchange mechanisms.
- Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma proteins.
- Simple operation with a syringe, a pump or a chromatography system such as AKTA design
- Stable over pH 5 to 10 and tolerates all commonly used aqueous buffers.
Stockage et stabilité
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Remarque sur l'analyse
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Autres remarques
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Informations légales
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
Certificats d'analyse (COA)
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