G8046

Anti-phospho-G3BP (pSer¹⁴⁹) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-G3BP1, Anti-GAP SH3 domain-binding protein, Anti-HDH-VIII, Anti-RAS-GTPase-activating protein SH3-domain binding protein 1, Anti-human DNA helicase VIII
• Numéro MDL: MFCD02262927
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~68 kDa
• Espèces réactives: human, rat (predicted), mouse (predicted)
• Concentration: ~1.0 mg/mL
• Technique(s): western blot: 5-10 μg/mL using whole extract of human K562 and HeLa cells
• Numéro d'accès UniProt: Q13283
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: phosphorylation (pSer149)
• Informations sur le gène: human ... G3BP1(10146) ; mouse ... G3bp1(27041) ; rat ... G3bp1(171092)

Description

Description générale

G3BP (Ras-GTPase-activating protein SH3 domain binding protein 1) is a 68kDa, single-strand-specific endoribonuclease that is phosphorylation-dependent.

Application

Anti-phospho-G3BP (pSer¹⁴⁹) antibody produced in rabbit is suitable for western blotting at a working concentration of 5-10μg/mL using whole extract of human K562 and HeLa cells.

Actions biochimiques/physiologiques

G3BP (Ras-GTPase-activating protein SH3 domain binding protein 1) exclusively cleaves between cytosine and adenine (CA). In dividing cells, G3BP interacts with RasGAP, linking between a RasGAP-mediated signaling pathway and RNA turnover. It is a RNA-binding protein that contains a carboxyl-terminal RNA binding domain, the RRM-type domain. It also has an amino-terminal domain homologous to nuclear transporter factor 2 (NTF2), and a central domain rich in acidic residues. The RRM domain is involved in the binding of G3BP to specific RNA sequences. A Ser149 residue is present 20 amino acids C terminal to the NTF2-like domain. Phosphorylation of this residue is crucial in mediating protein-protein interactions and in controlling the subcellular localization of G3BP. G3BP facilitates the assembly of stress granules that are invoved in the regulation of mRNA metabolism during stress. Dephosphorylation of Ser149 results in oligomerization and SG assembly. Overexpression of G3BP is observed in malignant tumors such as lung cancer, colon cancer, gastric cancer, and breast cancer. The level of G3BP expression in breast cancer specimens is positively correlated to the presence of lymph node metastasis.

Description de la cible

G3BP (pSer¹⁴⁹) encodes one of the DNA-unwinding enzymes which prefers partially unwound 3′-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion.

Forme physique

Solution 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)