G1163
O-Glycosidase from Streptococcus pneumoniae
recombinant, expressed in E. coli, buffered aqueous solution
Synonyme(s) :
Endo-α-N-acetylgalactosaminidase, O-Glycanase
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About This Item
Produits recommandés
Produit recombinant
expressed in E. coli
Niveau de qualité
Conjugué
(O-linked)
Forme
buffered aqueous solution
Poids mol.
180 kDa
Concentration
≥800 units/mL
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Actions biochimiques/physiologiques
Releases unsubstituted Ser- and Thr-linked β-Gal-(1→3)-α-GalNAc (Core 1 type O-glycan) from glycoproteins. Substitutions of the disaccharide core with sialic acid, lactosamine (galactose-N-acetyl glucosamine), or fucose will block hydrolysis and prevent the liberation of the oligosaccharide from the protein. Pretreament with glycolytic enzymes to remove substituent saccharides from the O-glycan may be needed prior to cleavage using O-glycosidase..
Conditionnement
Supplied with 5× Reaction Buffer, 250 mM NaH2PO4 pH 5.0.
Définition de l'unité
One unit will hydrolyze 1 μmole of p-nitrophenyl galacto-N-bioside (β-Gal-(1→3)-α-GalNAc-1→ΟC6H4NO2) per min at 37 °C at pH 6.5.
Forme physique
Solution in 50 mM sodium phosphate, pH 7.5
Remarque sur l'analyse
Screened for presence of: β-galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, neuraminidase, and proteases. See Certificate of Analysis for lot specific information.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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