FLAGIPT1
FLAG® Immunoprecipitation Kit
Synonyme(s) :
Anti-ddddk, Anti-dykddddk
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
Changer de vue
A propos de cet article
NACRES:
NA.32
UNSPSC Code:
12352200
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderusage
kit sufficient for 50 reactions
Quality Level
analyte chemical class(es)
proteins (FLAG® Affinity Gels)
technique(s)
immunoprecipitation (IP): suitable
shipped in
wet ice
storage temp.
−20°C
General description
Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.
Application
Learn more product details in our FLAG® application portal.
Features and Benefits
- Utilizes highly specific ANTI-FLAG M2 affinity gel
- No preliminary steps or calibrations
- 3xFLAG™ peptide provides efficient and gentle elution through direct competition
Other Notes
All prodedures to be performed at 2-8°C.
Legal Information
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Classe de stockage
10 - Combustible liquids
wgk
WGK 3
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Contenu apparenté
Konstantinos Koudounas et al.
Plant physiology, 174(3), 1371-1383 (2017-05-10)
Oleuropein, a terpene-derived glycosylated secoiridoid biosynthesized exclusively by members of the Oleaceae family, is involved in a two-component defense system comprising a β-glucosidase that activates oleuropein into a toxic glutaraldehyde-like structure. Oleuropein and its deglycosylated derivatives have high pharmaceutical interest.
Kuancan Liu et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 28(3), 901-913 (2020-01-29)
Esophageal squamous cell carcinoma (ESCC) is a predominant cancer type in developing countries such as China, where ESCC accounts for approximately 90% of esophageal malignancies. Lacking effective and targeted therapy contributes to the poor 5-year survival rate. Recent studies showed
Mercedes Rodriguez-Teja et al.
Molecular cancer research : MCR, 13(3), 538-547 (2014-11-09)
Epithelial cell-cell contacts maintain normal glandular tissue homeostasis, and their breakage can trigger epithelial-to-mesenchymal transition (EMT), a fundamental step in the development of metastatic cancer. Despite the ability of C-type lectin domains (CTLD) to modulate cell-cell adhesion, it is not
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| FLAGIPT1-1KT | 04061833622902 |