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F9291

Sigma-Aldrich

Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Synonyme(s) :

Anti-ddddk, Anti-dykddddk

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.32

Source biologique

mouse

Conjugué

biotin conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

M2, monoclonal

Forme

buffered aqueous glycerol solution

Espèces réactives

all

Concentration

~1 mg/mL

Technique(s)

dot blot: suitable (chemiluminescent detection)

Isotype

IgG1

Séquence immunogène

DYKDDDDK

Conditions d'expédition

dry ice

Température de stockage

−20°C

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Description générale

The monoclonal Anti-FLAG BioM2 mouse antibody is covalently attached to biotin by hydrazide linkage. The antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus or C-terminus of FLAG fusion porteins.

Application

Biotin-labeled antibody is used for immunodetection methods using avidin- or streptavidin-conjugated reporter enzymes such as streptavidin-peroxidase. Primary antibody conjugates are preferred when using murine cells as the recombinant protein host.
Antibody is suitable for immunofluorescence, western blotting, microscopy applications and for the formation of avidin-biotin complexes.

Learn more product details in our FLAG® application portal.

Forme physique

Solution in 50% glycerol, 10 mM sodium phosphate, pH 7.25, containing 150 mM NaCl and 0.02% sodium azide

Notes préparatoires

Dilute antibody in TBS (.05M Tris, pH7.4, with .15M NaCl) to a final concentration of 1-10μg/mL.

Informations légales

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Yingru Liu et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 30(6), 2025-2038 (2010-02-12)
To assess the effects and mechanisms of a CD200R1 agonist administered during the progressive stage of a multiple sclerosis model, we administered CD200R1 agonist (CD200Fc) or control IgG2a during the chronic phase of disease (days 10-30) in mice with experimental
Hong-Won Lee et al.
Nature communications, 4, 1505-1505 (2013-02-21)
Co-immunoprecipitation (co-IP) has become a standard technique, but its protein-band output provides only static, qualitative information about protein-protein interactions. Here we demonstrate a real-time single-molecule co-IP technique that generates real-time videos of individual protein-protein interactions as they occur in unpurified
Nawal Bendris et al.
PloS one, 6(7), e22879-e22879 (2011-08-11)
Cyclin A2 is essential at two critical points in the somatic cell cycle: during S phase, when it activates CDK2, and during the G2 to M transition when it activates CDK1. Based on the crystal structure of Cyclin A2 in
Helen Hwang et al.
Scientific reports, 4, 6391-6391 (2014-09-30)
The ends of eukaryotic chromosomes are capped by telomeres which consist of tandem G-rich DNA repeats stabilized by the shelterin protein complex. Telomeres shorten progressively in most normal cells due to the end replication problem. In more than 85% of
Qing Xu et al.
Nature communications, 8(1), 425-425 (2017-09-06)
Tumor necrosis factor (TNF) has a critical role in diverse cellular events including inflammation, apoptosis and necroptosis through different signaling complexes. However, little is known about how the transition from inflammatory signaling to the engagement of death pathways is modulated.
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