F3133

Fibroblast Growth Factor from bovine pituitary

lyophilized, suitable for cell culture

• Synonyme(s) : FGF
• Numéro CAS: 62031-54-3
• Numéro MDL: MFCD00166724
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• product name: Fibroblast Growth Factor from bovine pituitary, suitable for cell culture
• Source biologique: bovine pituitary glands
• Niveau de qualité: 200
• Forme: lyophilized powder
• Puissance: 1-100 ng/mL
• Poids mol.: 16.4 kDa
• Conditionnement: pkg of 10 and 5X10 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Couleur: white to off-white
• Température de stockage: −20°C

Description

Description générale

Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface. There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34kDa and they possess a conserved sequence of 120 amino acids.

Application

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.

Fibroblast Growth Factor from bovine pituitary has been used in in vitro testicular cell culture. It has also been used as a component of myogenic culture medium.

Actions biochimiques/physiologiques

Fibroblast growth factors (FGF) actively participate in cellular responses including embryogenesis and organogenesis. It specifically controls cell proliferation, differentiation and migration. FGF is known to regulate nervous system functions, tissue repair mechanism and wound healing. It also controls angiogenesis of tumor cells. Abnormal FGF mediated signaling induces tumor angiogenesis, by stimulating cancer cell proliferation and survival. FGFs carries out its function by mediating through four receptor tyrosine kinases : FGF-receptors FGFR1, FGFR2, FGFR3 and FGFR4.

Qualité

Although the major mitogenic component of this product is FGF2, the activity per unit weight of protein is less than that of purified FGF2.

Forme physique

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.1 mg/ml BSA.

Notes préparatoires

Purified glands by a modification of the method of Gospodarowicz.

Remarque sur l'analyse

The biological activity is measured using a 3-day MTT assay.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)