F0772
Monoclonal Anti-CD8−FITC antibody produced in mouse
clone UCHT-4, purified immunoglobulin, buffered aqueous solution
Synonyme(s) :
Monoclonal Anti-CD8
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.44
Produits recommandés
Source biologique
mouse
Conjugué
FITC conjugate
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
UCHT-4, monoclonal
Forme
buffered aqueous solution
Espèces réactives
human
Technique(s)
flow cytometry: 10 μL using 1 × 106 cells
Isotype
IgG2a
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
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Description générale
Monoclonal Anti-Human CD8 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from Balb/c mice immunized with human thymocytes followed by peripheral blood T cells. The CD8 antigen is strongly expressed on approximately one-third of mature T cells (cytotoxic/suppressor T cells). Monoclonal Anti-CD8 does not stain B lymphocytes, monocytes, or granulocytes.
Spécificité
Recognizes the CD8 human T cytotoxic/suppressor lymphocyte cell surface glycoprotein. Approximately 90% of thymocytes are stained in suspension. In thymus sections, both cortical and medullary thymocytes are stained. A subset of NK cells express this antigen weakly. The epitope recognized by this clone is sensitive to routine formalin fixation and paraffin embedding.
3rd Workshop: code no. 567
3rd Workshop: code no. 567
Immunogène
human thymocytes followed by peripheral blood T cells.
Application
FITC Monoclonal Anti-Human CD8 may be used for:
- Immunofluorescent staining,
- Enumeration of total T cytotoxic/suppressor lymphocytes in bone marrow, blood and other body fluids,
- Identification and localization of T cytotoxic/ suppressor lymphocytes in lymphoid and other tissues,
- Analysis of cell mediated cytotoxicity,
- Studies of immunoregulation in health and disease,
- Investigation of NK cells, Complement mediated cytolysis of CD8 expressing cells
Actions biochimiques/physiologiques
CD8a (Cluster of Differentiation 8a) is a human gene. CD8 bind to class I MHC and the co-receptors during the process of cytotoxic T-cell activation. T cells that express T cell receptor (TCR) complexes, which lack CD3 δ, exhibit severely impaired positive selection and TCR-mediated activation of CD8 single-positive T cells. CD3 δ couples with TCR. The CD3 associated with CD8 is required for effective activation and positive selection of CD8+ T cells. CD8 expression is a recurrent rare phenomenon in patients with chronic lymphocytic leukemia (CLL).
Description de la cible
CD8 is a two chain complex (α/α, α/β) receptor for Class I MHC molecules. The receptor′s cytoplasmic portion is associated with p56lck protein tyrosine kinase which phosphorylates nearby proteins. The antigen is strongly expressed on approximately one third of mature T cells (cytotoxic/suppressor T cells).
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Notes préparatoires
Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Christian Schütz et al.
Blood, 111(7), 3546-3552 (2007-12-22)
Several cell-based immunotherapy strategies have been developed to specifically modulate T cell-mediated immune responses. These methods frequently rely on the utilization of tolerogenic cell-based antigen-presenting cells (APCs). However, APCs are highly sensitive to cytotoxic T-cell responses, thus limiting their therapeutic
CXCL9 promotes prostate cancer progression through inhibition of cytokines from T cells
Tan S, et al.
Molecular Medicine Reports, 18(2), 1305-1310 (2018)
G F Gao et al.
Immunology today, 21(12), 630-636 (2000-12-15)
In recent years, substantial progress has been made towards understanding the molecular basis for CD8 binding to class I MHC and the coreceptor's role in cytotoxic T-cell activation. Here, we review the structural, mechanistic and functional studies that point to
Wolfgang Kern et al.
Cytometry. Part B, Clinical cytometry, 82(3), 145-150 (2011-12-07)
In patients with chronic lymphocytic leukemia (CLL), aberrant expression of the T-lineage antigen CD8 was reported in low frequencies. The clinical impact of this phenomenon remains in discussion. We analyzed 5,523 patients with CLL (21-97 years) by multiparametric flow cytometry
Marie-Agnès Doucey et al.
The Journal of biological chemistry, 278(5), 3257-3264 (2002-09-07)
T cells expressing T cell receptor (TCR) complexes that lack CD3 delta, either due to deletion of the CD3 delta gene, or by replacement of the connecting peptide of the TCR alpha chain, exhibit severely impaired positive selection and TCR-mediated
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