Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
E5134
Acide éthylènediaminetétraacétique disodium salt dihydrate
suitable for electrophoresis, for molecular biology, 99.0-101.0% (titration)
Synonyme(s) :
Séquestrène Na₂, EDTA disodium salt, EDTA-Na2, Edétate disodium salt dihydrate, Sequestrene Na2, Édathamil, Éthylènediaminetétraacétate disodique dihydrate
About This Item
Produits recommandés
Qualité
Molecular Biology
for molecular biology
Essai
99.0-101.0% (titration)
Forme
powder
Pertinence de la réaction
reagent type: chelator
Technique(s)
electrophoresis: suitable
Impuretés
≤0.005% Insolubles
≤0.1% Nitrilotriacetic acid (NTA)
Pf
248 °C (dec.) (lit.)
Solubilité
3 M NaOH: 1.6 g/10 mL
Traces de cations
Fe: ≤0.005%
Pb: ≤0.002%
Adéquation
suitable for electrophoresis
suitable for molecular biology
Activité étrangère
DNase, RNase, NICKase and protease, none detected
Température de stockage
room temp
Chaîne SMILES
O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
InChI
1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2
Clé InChI
OVBJJZOQPCKUOR-UHFFFAOYSA-L
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Description générale
Application
- comme constituant de solutions tampons pour l'électrophorèse, la culture cellulaire, des essais enzymatiques, l'analyse de petits ARN interférents, la cytométrie en flux, l'analyse au bleu de diméthylméthylène, etc.[2].
- comme anticoagulant pour le prélèvement de sang total[1].
- comme solution stabilisante pour des échantillons de culture cellulaire congelés[3].
- en association avec du PBS et de la trypsine, pour trypsiniser des cellules[4].
- pour la phytoextraction de métaux lourds dans des sols pollués.[5]
Caractéristiques et avantages
- Qualité "biologie moléculaire", convenant à de nombreuses applications de biologie moléculaire.
- Produit exempt de DNases, RNases, Nickases et protéases.
Autres remarques
Produit comparable
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Inhalation - STOT RE 2 Inhalation
Organes cibles
Respiratory Tract
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Certificats d'analyse (COA)
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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
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Les clients ont également consulté
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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Product E5134 has a re-test date of Aug. 2023. Has the re-test been completed?
1 answer-
The specific lot number is required to determine the retest status. You may obtain this lot status by contacting our Technical Service team. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquires" under the Products Section with all the required information, including the specific lot number, so that a member of our team can reach out to you to assist further. Thank you.
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Can solutions of Product E5134, EDTA disodium salt, be autoclaved?
1 answer-
Yes.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the working concentration for Product E5134, EDTA disodium salt, for use as an anti-coagulant?
1 answer-
The optimal concentration is 1.5 mg per mL of blood.
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Is Product E5134, EDTA disodium salt, stable in solution?
1 answer-
Yes. A 0.5 M stock solution at pH 8.5 is stable for months at 2-8°C.
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