E2884
Esterase from porcine liver
ammonium sulfate suspension, ≥150 units/mg protein (biuret)
Synonyme(s) :
Carboxyl esterase, Carboxylic-ester hydrolase, PLE
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About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
Produits recommandés
Source biologique
Porcine liver
Niveau de qualité
Forme
ammonium sulfate suspension
Activité spécifique
≥150 units/mg protein (biuret)
Poids mol.
168 kDa
Température de stockage
2-8°C
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Description générale
Esterase, also known as carboxyl ester hydrolase is a member of the hydrolase superfamily of enzymes.
Application
Esterase from porcine liver has been used to unmask the fluorescence in ultraviolet-visible spectroscopy.
The enzyme from Sigma has been used to develop an alternative gel-based protocol for screening for bead-bound catalytic activity in aqueous media. The product has also been used to investigate the properties of the compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4, an aminoacid ester prodrug of the adenosine A2A receptor antagonist MSX-2).
Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications.
Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.
Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.
Actions biochimiques/physiologiques
Esterase acts on water-soluble carboxyl esters containing short chain fatty acids. Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu.
Esterase enzyme of bacteria and fungi seems to have several industrial applications. It is capable of catalyzing the hydrolysis and synthesis of ester bonds.
Pig liver esterase catalyzes enantioselective conversion of an ester to a carboxylic acid. The molecular weight is found to be 168 kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half-molecules in the presence of dilute acid or concentrated salts.
Définition de l'unité
One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.
Forme physique
Suspension in 3.2 M (NH4)2SO4, pH 8
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificats d'analyse (COA)
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Les clients ont également consulté
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Molecules (Basel, Switzerland), 13(2), 348-359 (2008-02-29)
The compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4) was synthesized as an amino acid ester prodrug of the adenosine A2A receptor antagonist MSX-2. It was found to be stable in artificial gastric acid, but readily cleaved by pig liver esterase.
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