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D6046

DMEM - low glucose

With sodium bicarbonate, sodium pyruvate and ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonyme(s) :

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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A propos de cet article

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
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Nom du produit

Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose, L-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Segment

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: yes
sodium pyruvate: yes
phenol red: yes
NaHCO3: yes
HEPES: no
glucose: low

shipped in

ambient

storage temp.

2-8°C

General description

This DMEM-Low glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.

Application

Dulbecco′s Modified Eagle′s Medium-low glucose is a fully supplemented liquid form of the original Dulbecco′s formulation, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium.
Dulbecco′s Modified Eagle′s Medium - low glucose has been used in the maintenance/culturing of mammalian cells.[1]

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Cet article
FG0415D5921D9443
sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: yes
sodium pyruvate: yes
phenol red: yes
NaHCO3: yes
HEPES: no
glucose: low

components

glucose: low
HEPES: no
sodium pyruvate: yes
NaHCO3: yes
stable glutamine: yes
phenol red: yes

components

phenol red: no
sodium pyruvate: no
glucose: low
HEPES: no
L-glutamine: no
NaHCO3: yes

components

NaHCO3: 3.7 g/L
sodium pyruvate: no
glucose: 1.0 g/L
L-glutamine: 0.584 g/L
phenol red: no
HEPES: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C


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Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–3 of 3 Questions  
  1. Does your low glucose DMEM (Model No.: D6046) contain vitamin D or vitamin A? I look forward to your reply.

    1 answer
    1. This product does not contain Vitamin D or Vitamin A.

      Helpful?

  2. Is DMEM a suitable media for growth of human dermal papilla cells, human fibroblast cells

    1 answer
    1. The formulation of this medium is detailed in the table at the link below. Please refer to the supplier of the cells and/or the published literature for details regarding the media requirements for the exact cell types and model systems in question.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/223/143/d6046for.pdf

      Helpful?

  3. D6046ですがラベルの表記にはピルビン酸が記載されていないにも関わらずWeb上ではピルビン酸を含むとなっています。 大抵の商品はピルビン酸を含む場合、ラベルに明記してあります。入っているのが正しいのでしょうか?

    1 answer
    1. Yes, this product contains 0.11 g/L of pyruvic acid. Please see the chart below that lists of the components for this product:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/223/143/d6046for.pdf

      Helpful?

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