D5523

Dulbecco′s Modified Eagle′s Medium - low glucose

With 1000 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

• Synonyme(s) : DME, DMEM
• UNSPSC Code: 12352207
• NACRES: NA.75
• MDL number: MFCD00217342

Propriétés

• Quality Segment: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: L-glutamine: yes; NaHCO₃: no; sodium pyruvate: yes; phenol red: yes; HEPES: no; glucose: low
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME) that comprises a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types.

Application

Dulbecco′s Modified Eagle′s medium-low glucose has been used in the differentiation and culture of human and murine mesenchymal stem cells (MSCs).

Preparation Note

Formulated to contain 10.0 grams of powder per liter of medium.

Supplement with 3.7 g/L sodium bicarbonate.

Informations sur la sécurité

• pictograms: GHS07
• signalword: Warning
• hcodes: H317,H319
• pcodes: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Sens. 1
• Classe de stockage: 13 - Non Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable