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D4668

Sigma-Aldrich

Dextranase from Penicillium sp.

lyophilized powder, 100-250 units/mg protein

Synonyme(s) :

1,6-α-D-Glucan 6-glucanohydrolase

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About This Item

Numéro CAS:
9025-70-1
Numéro de classification (Commission des enzymes):
3.2.1.11 (BRENDA, IUBMB)
Numéro CE :
232-803-9
Numéro MDL:
MFCD00130940
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Forme

lyophilized powder

Niveau de qualité

200

Activité spécifique

100-250 units/mg protein

Composition

Protein, ~25% Lowry

Température de stockage

2-8°C

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Application

Dextranase from Penicillium sp. can be used to modify alternan. Dextranase from Penicillium sp. has also been used in a study that explored the possibility that the Dex protein might adopt a fold unfamiliar to any known structure.

Actions biochimiques/physiologiques

The product is an endodextranase that hydrolyzes α-(1,6)-glucosidic linkages in dextran. Dextrans are undesirable compounds synthesized from sucrose by microbial contaminants during sugar production. They increase viscosity of the flow and decrease industrial recovery. Dextranase has been used for hydrolyzing dextran at sugar mills in order to improve efficiency of sugar production.

Définition de l'unité

One unit will liberate 1.0 μmole of isomaltose (measured as maltose) per min at pH 6.0 at 37 °C, using dextran as substrate.

Forme physique

Partially purified, lyophilized powder

Autres remarques

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

T Pons et al.
Proteins, 31(4), 345-354 (1998-06-17)
The DEX gene encodes an extracellular dextranase (EC 3.2.1.11); this enzyme hydrolyzes the alpha(1,6) glucosidic bond contained in dextran to release small isomaltosaccharides. Sequence analysis has revealed only one homologous sequence, CB-8 protein, from Arthrobacter sp., with 30% sequence identity.
Timothy D Leathers et al.
Biotechnology letters, 31(2), 289-293 (2008-10-22)
Alternan is a unique glucan with a backbone structure of alternating alpha-(1 --> 6) and alpha-(1 --> 3) linkages. Previously, we isolated strains of Penicillium sp. that modify native, high molecular weight alternan in a novel bioconversion process to a
Controlled nanoparticle formation by enzymatic deshelling of biopolymer-stabilized nanosuspensions.
Yi-Yeoun Kim et al.
Small (Weinheim an der Bergstrasse, Germany), 5(8), 913-918 (2009-02-11)
Hongbin Zhang et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 51(4), 495-503 (2011-07-30)
To obtain new fungi producing dextranase,we screened and identified a strain F1001 showing high dextranase activities. We provided a new strain with dextranase activity for producing clinical dextran. Morphological and ITS rDNA sequences homology analysis were performed to identify the
Akira Yano et al.
Applied microbiology and biotechnology, 86(2), 615-623 (2009-11-11)
Mushrooms contain large quantities of alpha-glucans. Shiitake (Lentinula edodes), Japan's most popular edible mushroom, has been reported to contain about 6% (weight/dried weight) of alpha-(1,3)-glucan. This glucan is one of the major components of oral biofilm formed by the cariogenic
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