CS0720
Citrate Synthase Assay Kit
1 kit sufficient for 100 reactions (using a 1 ml cuvette), 1 kit sufficient for 480 reactions (using 96 multiwell plates)
Synonyme(s) :
Citrate Synthase Activity Test Kit
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.28
Produits recommandés
Description générale
The Citrate Synthase Assay Kit contains all the required reagents (including a positive control enzyme) for a fast and simple measurement of citrate synthase activity in a whole cell extract or in isolated mitochondria. In addition, the kit enables testing of the intactness of the mitochondrial inner membrane.
Actions biochimiques/physiologiques
Citrate synthase is the initial enzyme of the tricarboxylic acid (TCA) cycle. The enzyme catalyzes the reaction of 2 carbon acetyl CoA with 4 carbon oxaloacetate to form the 6 carbon citrate. This enzyme is an exclusive marker of the mitochondrial matrix.
Remarque sur l'analyse
The activity of the enzyme is measured by following the color of TNB, which is generated from DTNB present in the reaction of citrate synthesis, and caused by the deacetylation of Acetyl-CoA. The overall reaction product, TNB, absorbs at 412 nm.
Composants de kit seuls
Réf. du produit
Description
- Assay buffer for citrate synthase 5x 25 mL
- Bicine buffer 10 mL
- CelLytic™ M Cell Lysis Reagent 10 mL
- DTNB [5,5'-Dithiobis-(2-nitrobenzoic acid)] 4 mg
- Acetyl coenzyme A 25 mg
- Oxaloacetic acid 1 g
- Citrate synthase (positive control) .1 mL
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
10 - Combustible liquids
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