CS0030

Senescence Cells Histochemical Staining Kit

sufficient for 100 tests

• Synonyme(s) : Senescent cells IHC kit, cellular β-galactosidase probe
• NACRES: NA.32
• UNSPSC Code: 12352207

Propriétés

• usage: sufficient for 100 tests
• Quality Segment: 200
• packaging: pkg of 1 kit
• storage condition: dry at room temperature
• detection method: colorimetric
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

The Senescence Cells Histochemical Staining Kit contains all the reagents required for identifying senescent cells using an assay based on a histochemical stain for β-galactosidase activity at pH 6. β-galactosidase activity is detectable in senescent cells, but not in quiescent, immortal, or tumor cells. Cellular senescence is a progression of events, whereby cells move from an actively dividing to a non-dividing stage. The cellular senescence process is associated with aging. The decrease in cell division is virtually irreversible and complete. In conjunction with the loss of the ability to divide, changes occur in the morphology, shape, and physical appearance of the cells, and their pattern of gene expression. At the end of the process cell death usually occurs, although the cells may remain viable for a long time.

Application

Senescence Cells Histochemical Staining Kit has been used for:

• senescence-associated β-galactosidase assay
• to test for senescence in human pancreatic cancer cells
• adipose-derived stem cells (ADSCs)
• cord blood mesenchymal stromal/stem cells (CBMSC)

Biochem/physiol Actions

Replicative senescence is a growth-arrest state associated with loss of division potential, changes in cell morphology, shape and physical appearance, and the pattern of gene expression in cells.

Legal Information

Manufactured under license to US Patent Nos. 5,491,069 and 5,795,728.

Composants de kit seuls

• X-gal solution 4 mL
• Staining Solution, 10X 15 mL
• Fixation Buffer 10x 15 mL
• Reagent B 1.5 mL
• Reagent C 1.5 mL
• Dulbecco's Phosphate Buffered Saline (PBS) 10x 60 mL

Informations sur la sécurité

• pictograms: GHS06,GHS08,GHS05
• signalword: Danger
• hcodes: H302 + H312,H314,H317,H331,H334,H335,H341,H350,H412
• pcodes: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• target_organs: Respiratory system
• supp_hazards: EUH032
• Classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects