b.End5

96091930, mouse brain, Endothelial-like

• Synonyme(s) : Brain-derived Endothelial 5 Cells, bEnd3 Cells, bEnd5 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: b.End5, 96091930
• Source biologique: mouse brain
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Endothelial-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse Balb/c brain endothelioma

Description de la lignée cellulaire

The cell line b.End5 has been established from brain endothelial cells of BALB/c mice. Immortalisation has been carried out by infection of primary cells with retrovirus coding for the Polyoma virus middle T-antigen. b.End5 cells are positive for endothelial specific proteins (PECAM-1, Endoglin, MECA-32, Flk-1) tested by FACS. Inflammatory cytokines induce the expression of proteins such as CAM-1, VCAM-1 and E-selectin. Injection into mice causes development of haemangiomas.

Application

Study of endothelial cell growth, differentiation and influence of oncogenes.

Milieu de culture

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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