LC-2/ad

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94072247, human lung, Epithelial

• Synonyme(s) : LC-2/ad Cells
• Code UNSPSC : 41106514

Propriétés

• Source biologique: human lung
• Conditionnement: tube of 5 μg 94072247-DNA-5UG; pkg of vial of cells 94072247-1VL
• Mode de croissance: Adherent
• Caryotype: Modal No 53-56
• Morphologie: Epithelial
• Produits: alpha-1-anti-trypsin
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human lung adenocarcinoma

Description de la lignée cellulaire

Established from the pleural effusion of a pulmonary adenocarcinoma in a 51 year-old Japanese female. LC-2/ad cells exhibit an epithelial appearance and a tendency to form small domes. Immunocytochemically the cells are positive for CEA and cytokeratins, including cytokeratin No.18. LC-2/ad produces at least two functionally active trypsin inhibitors together with several proteinases in vitro.

Application

LC-2/ad has been used to study the ability of RXDX-105 as a therapy for cancers which are affected by RET (proto-oncogene tyrosine-protein kinase receptor) changes.

Milieu de culture

RPMI 1640:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Procédure de repiquage

Split confluent cultures 1:2-1:4 ie seeding at 2-5 x 10,000 cells/cm² using 0.25% trypsin or trypsin/ EDTA ; 5%CO₂; 37°C

Autres remarques

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