A10-85

93040117, rat nerve, Epithelial-like

• Code UNSPSC : 41106514

Propriétés

• product name: A10-85, 93040117
• Source biologique: rat nerve
• Mode de croissance: Adherent
• Caryotype: Modal no. 42
• Morphologie: Epithelial-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat glioma

Description de la lignée cellulaire

The glioma cell line A10-85 is a subclone of A10 cells which originally emerged from the parent line IA2 by reducing the serum concentration from 15% to 10%. This parent line IA2 was derived from a rat brain glioma induced transplacentally by ethylnitroso urea (inbred BDIxrats). A10-85 cells form processes in response to cAMP. GAPDH can be induced by hydrocortisone succinate and good growth was obtained in semi-solid medium. Injection of cells into syngeneric animals resulted in the formation of malignant astrocytic tumours after short latent periods.

Application

Experimental tumour models, carcinogenesis studies

Milieu de culture

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:50 to 1:100 seeding at 5x10,000 to 1x100,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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