HT29 Cell Line human

91072201, human colon, Epithelial

• Synonyme(s) : HT29 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: HT29 Cell Line human, 91072201, Caucasian colon adenocarcinoma grade II
• Source biologique: human colon
• Mode de croissance: Adherent
• Caryotype: 2n = 46, hypertriploid
• Morphologie: Epithelial
• Produits: Secretory component of Immunoglobulin A (IgA), Carcinoembryonic antigen (CEA)
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian colon adenocarcinoma

Description de la lignée cellulaire

Isolated from a primary tumour in a 44 year old Caucasian female. Forms a well-differentiated adenocarcinoma consistent with colony primary, grade I. Tumours also form in steroid treated hamsters. Has the following HLA profile A1,3; B12,17; Cw5.

Application

HT29 Cell Line has been used to determine viral titres of human parechovirus. It has also been used to study the ability of Bifidobacterium and Lactobacillus strains to counteract the toxic effect of C. difficile LMG21717.

Tumourigenicity studies

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19

Milieu de culture

McCoy′s 5A + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% Trypsin; 5% CO₂; 37°C.

Autres remarques

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