RTG-2

90102529, fish ovary, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: RTG-2, 90102529
• Source biologique: fish ovary
• Mode de croissance: Adherent
• Caryotype: 2n = 60
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Trout Rainbow gonad tissue

Description de la lignée cellulaire

From pooled male/female gonad tissue of yearling rainbow trout (Oncorhynchus mykiss). First cell line to be established from cold-blooded vertebrates. 8-fold difference in growth rate in temperature range 4-24°C. Susceptible to infectious pancreatic necrosis virus, Egtved virus and arboviruses (EEE, VEE).

Application

Virus studies: Inf. pacr. necrotic virus, Egtved virus, arboviruses (EEE, VEE)

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Passage using 0.25% trypsin below 24°C. Cells may be seeded as few as 3x10,000 cells/ml 5% CO₂; 24°C. Fish cell lines detach easily during transit if the culture is very youn

Autres remarques

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