R2C

89031606, rat testis, Epithelial

• Code UNSPSC : 41106514

Propriétés

• Nom du produit: R2C, 89031606
• Source biologique: rat testis
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial
• Produits: Steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat testis Leydig cell tumour

Description de la lignée cellulaire

A rat testis Leydig cell tumour cell line derived from a 2-month-old male WFu rat. Virus susceptibility: herpes simplex; vaccinia; pseudorabies; vesicular stomatitis (Indiana). Resistant to poliovirus 2. Stimulation by other hormones or cAMP is not required for hormone secretion.

Milieu de culture

HAMS F10 + 2mM Glutamine + 15% Horse serum + 2.5% Foetal Bovine Serum (FBS). Do not use heat-inactivated serum.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA;5% CO₂; 37°C.

Autres remarques

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