AtT20

87021902, mouse brain, Small round cells growing as large aggregates

• Code UNSPSC : 41106514

Propriétés

• product name: AtT20, 87021902
• Source biologique: mouse brain
• Mode de croissance: Aggregates in suspension
• Caryotype: Diploid
• Morphologie: Small round cells growing as large aggregates
• Produits: Adrenocorticotropic hormone (ACTH)
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse LAF1 pituitary gland tumour

Description de la lignée cellulaire

Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.

Application

Hormone studies

Milieu de culture

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates.

Procédure de repiquage

Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding densi

Autres remarques

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