Vero C1008 [Vero 76, clone E6, Vero E6]

85020206, African green monkey kidney, Epithelial

• Synonyme(s) : Vero 76 Clone E6 Cells, Vero Cells, Vero E6 Cells, VeroC1008 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: Vero C1008 [Vero 76, clone E6, Vero E6], 85020206, from African green monkey kidney, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• Source biologique: African green monkey kidney
• Mode de croissance: Adherent
• Caryotype: 2n = 60
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description générale

Vero cells are derived from the kidney of an African green monkey. These are anchorage-dependent cells that have applications in molecular and cell biology research. Vero E6 cells enable achieving high titers of severe acute respiratory syndrome coronavirus (SARS-CoV virus) and are also used in the cultivation of Rabies virus, Reovirus and Japanese encephalitis virus.

Origine de la lignée cellulaire

Monkey African Green kidney

Description de la lignée cellulaire

A subclone of Vero76 (Sigma catalog no. 85020205) exhibiting a similar range of virus susceptibility (haemorrhagic viruses). They show some degree of contact inhibition and are suitable for supporting the growth of slowly replicating viruses.

Application

VERO C1008 has been used for transfection, cultivation and propagation of West Nile virus, yellow fever virus, ZIKA, Ebola virus, Sabethes flavivirus (SbFV), Crimean-Congo Haemorrhagic Fever (CCHF), Human-derived Lake Victoria Marburg virus strain Popp.

Milieu de culture

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Autres remarques

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