C8093

Monoclonal Anti-Connexin-43 antibody produced in mouse

clone CXN-6, ascites fluid

• Synonyme(s) : Mouse monoclonal antibody
• Numéro MDL: MFCD00803532
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Conjugué: unconjugated
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: CXN-6, monoclonal
• Poids mol.: antigen 43 kDa
• Contient: 15 mM sodium azide
• Espèces réactives: rat, hamster, bovine, mouse, human
• Technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunohistochemistry (frozen sections): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1:8,000-1:16.000 using mouse whole brain extract
• Isotype: IgM
• Numéro d'accès UniProt: P17302
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... GJA1(2697) , GJA1P1(2698) ; mouse ... Gja1(14609) ; rat ... Gja1(24392)

Description

Description générale

Monoclonal Anti-Connexin-43 (mouse IgM isotype) is derived from the CXN-6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Connexin 43 (Cx43) is also known as GJA1 (gap junction protein alpha 1). The structure of connexin molecules includes a cytoplasmic N-terminal region, four transmembrane domains, two extracellular loops and a C-terminal cytoplasmic tail of varying length.

The 43 kDa connexin protein (connexin-43, Cx43) belongs to the a-type (group II) subfamily of connexin proteins. It is expressed in most tissues, even though the pattern of expression may differ in various cell types. For example, in the brain it is found in astrocytes, ependyma and leptomeninges but not in neurons, oligodendrocytes and pinealocytes; in the liver it is present in Ito cells but not hepatocytes.

Spécificité

Monoclonal Anti-Connexin-43 reacts specifically with connexin-43. Reactivity has been observed with human, bovine, hamster, rat, and mouse connexin-43.

Immunogène

synthetic connexin-43 peptide (362-381).

Application

Monoclonal Anti-Connexin-43 antibody produced in mouse has been used in:

• fluorescent immunostaining
• western blotting
• immunocytochemistry
• preparation of immuno-affinity columns

Actions biochimiques/physiologiques

Mutations in connexin 43 (Cx43) gene result in pleiotropic phenotype of oculodentodigital dysplasia. Connexin 43 participates in the multiplication and differentiation of mature T cells. It also participates in the secretion and manufacturing of cytokines. It helps to release lactate from glycolytic pancreatic ductal adenocarcinoma cells.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable