C3556
Catalase from human erythrocytes
≥90% (SDS-PAGE), buffered aqueous solution, ≥30,000 units/mg protein
Synonyme(s) :
H2O2:H2O2 oxidoreductase
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About This Item
Produits recommandés
Source biologique
human erythrocytes
Niveau de qualité
Pureté
≥90% (SDS-PAGE)
Forme
buffered aqueous solution
Activité spécifique
≥30,000 units/mg protein
Poids mol.
tetramer ~250 kDa
Concentration
≤10 mg/mL
Technique(s)
cell based assay: suitable
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Température de stockage
−20°C
InChI
1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
Clé InChI
NUVBSKCKDOMJSU-UHFFFAOYSA-N
Informations sur le gène
human ... CAT(847)
Description générale
Research area: Cell Signaling
Human catalase is a member of the monofunctional heme-containing catalases. It is an intracellular enzyme located at higher concentrations in the liver, erythrocytes, and kidney. Catalase is a homo-tetrameric protein and comprises amino acid residues, one heme group that is iron III protoporphyrin IX, and a nicotinamide adenine dinucleotide phosphate (NADPH) molecule. It is a ubiquitous enzyme found in most aerobic organisms. The catalase (CAT) gene is located on the human chromosome at 11p13.
Human catalase is a member of the monofunctional heme-containing catalases. It is an intracellular enzyme located at higher concentrations in the liver, erythrocytes, and kidney. Catalase is a homo-tetrameric protein and comprises amino acid residues, one heme group that is iron III protoporphyrin IX, and a nicotinamide adenine dinucleotide phosphate (NADPH) molecule. It is a ubiquitous enzyme found in most aerobic organisms. The catalase (CAT) gene is located on the human chromosome at 11p13.
Application
Catalase from human erythrocytes has been used:
- to prevent reoxidation of reduced cytochrome c by H2O2 while measuring the production of superoxide with cytochrome C.
- as a component of the imaging buffer for stochastic optical reconstruction microscopy (STORM) imaging of human skin fibroblasts
- as a component of the gloxy mix for single-molecule imaging
Actions biochimiques/physiologiques
Deficiency of catalase and oxidative stress are associated with vitiligo, hypertension, Alzheimer′s disease, diabetes mellitus, and acatalasemia. Catalase shows a dichotomous role in cancer as its expression is at a higher level in chronic lymphocytic leukemia, glioma, melanoma, and gastric cancer and lower levels of expression are seen in acute myeloid leukemia, pancreatic, lung, prostate, and skin (non-melanoma) cancers.
Catalase activates the decomposition of hydrogen peroxide, a reactive oxygen species, into water and oxygen. It functions as a natural antioxidant, protecting cells against oxidative damage to proteins, lipids and nucleic acids. Catalase has also been used to study the role reactive oxygen species play in gene expression and apoptosis.
Définition de l'unité
One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.
Forme physique
Solution in 50 mM Tris, pH 8.0
Stockage et stabilité
Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons
persons
Remarque sur l'analyse
Protein determined by biuret
Inhibiteur
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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