C-14110

Human CD14+ Monocytes (hMoCD14+-PB)

Isolated from peripheral blood, single donor, 1 million cells per pellet

• Code UNSPSC : 41106514
• Nomenclature NACRES : NA.81

Propriétés

• Source biologique: human peripheral blood
• Conditionnement: pkg of 1,000,000 cells
• Morphologie: (monocyte)
• Application(s): cell analysis (DNA, RNA, and protein)
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Cells are dissolved in RNAlater for subsequent RNA, DNA, or protein analysis. Note that cell pellets cannot be revived.

Origine de la lignée cellulaire

Blood

Application

Monocytes (Mo) are immature phagocytic cells circulating in the blood. Acting as antigen-presenting immune cells, they can phagocytize and degrade microbes and particulate matter. Monocytes can differentiate into several different cell types. The most common applications for Monocytes are the in vitro differentiation into monocyte-derived dendritic cells (moDC), macrophages, and osteoclasts as well as their use as phagocytic immune cells in immunology and infection biology.CD14 belongs to the family of LPS receptor antigens and is strongly expressed on the majority of Monocytes. Thus, binding of immunomagnetic particles to this antigen is employed for purification of PromoCell CD14+-Monocytes (hMoCD14+-PB) from Mononuclear Cells (MNC). Human CD14+ Monocytes are isolated from fresh peripheral blood. After isolation of the ultra-pure mononuclear cell fraction by proprietary methods, the CD14+ Monocytes are isolated by positive selection with >95% purity.

Qualité

Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Avertissement

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Forme physique

Pellet is dissolved in RNAlater^® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.

Procédure de repiquage

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Autres remarques

The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.

Informations légales

RNAlater is a registered trademark of Ambion, Inc.

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du Ministère français de la Recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable