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B3150

Sigma-Aldrich

Monoclonal Anti-Protein A−Biotin antibody produced in mouse

clone SPA-27, purified immunoglobulin, buffered aqueous solution

Synonyme(s) :

Monoclonal Anti-Protein A

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

mouse

Niveau de qualité

Conjugué

biotin conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

SPA-27, monoclonal

Forme

buffered aqueous solution

Technique(s)

direct ELISA: 1:80,000-1:160,000

Isotype

IgG1

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

Protein A is a cell wall associated protein isolated from Staphylococcus aureus Cowan 1 strain. The 42 kDa protein consists of a single polypeptide chain. Protein A possesses a specific affinity for Fc region of many mammalian immunoglobulins as human, rabbit, and guinea pig IgGs and therefore, considered as a universal reagent in biochemistry and immunology. Antibodies to protein A have been produced in rabbits, guinea pigs, chickens and mice. Protein A affinity chromatography has been achieved for the purification of immmunoglobulins. Monoclonal Anti-Protein A (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Spécificité

Specific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A.

Immunogène

protein A from Cowan I strain of Staphy­lococcus aureus.

Application

Monoclonal Anti-Protein A-Biotin antibody produced in mouse may be used in ELISA at a working dilution of 1:80,000 - 1:160,000.

Actions biochimiques/physiologiques

Protein A is an immunoglobulin-binding protein present on the surface of Staphylococcus aureus and is also secreted into the extracellular environment. It binds the Fc region of Igs and Fab regions of B cell receptors. The coupling of biotin to monoclonal Anti-Protein A antibody allows for the binding of various labels such as avidin or streptavidin.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.

Notes préparatoires

Prepared by conjugation of biotinamidocaproate N-hydroxysuccinimide ester to fractionated monoclonal anti-Protein A.

Stockage et stabilité

Store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Protein A chromatography: challenges and progress in the purification of monoclonal antibodies
Ramos-de-la-Pena AM, et al.
Journal of Separation Science (2019)
Evaluation of indirect enzyme-linked immunosorbent assays and IgG avidity assays using a protein A-peroxidase conjugate for serological distinction between Brucella abortus S19-vaccinated and-infected cows
Pajuaba ACAM, et al.
Clinical and Vaccine Immunology : CVI, 17(4), 588-595 (2010)
Single-domain protein A-engineered magnetic nanoparticles: toward a universal strategy to site-specific labeling of antibodies for targeted detection of tumor cells
Mazzucchelli S, et al.
ACS Nano, 4(10), 5693-5702 (2010)
Antibodies for Immunohistochemistry
Immunohistochemistry: Basics and Methods (2009)
Robert Prucek et al.
Scientific reports, 11(1), 6240-6240 (2021-03-20)
Targeted and effective therapy of diseases demands utilization of rapid methods of identification of the given markers. Surface enhanced Raman spectroscopy (SERS) in conjunction with streptavidin-biotin complex is a promising alternative to culture or PCR based methods used for such

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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