A9205

Albumine de sérum bovin solution

30% in saline, fatty acid free, aseptically filled

• Synonyme(s) : Albumine solution from bovine serum, Solution de BSA
• Numéro CAS: 9048-46-8
• Numéro MDL: MFCD00130384
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.27

Propriétés

• Source biologique: bovine
• Niveau de qualité: 200
• Stérilité: aseptically filled
• Pureté: 28-32% protein basis (biuret)
• Forme: solution
• Produit purifié par: cold ethanol fractionation
• Ne contient pas: stabilizer or preservative
• Origine: USA origin
• Concentration: 28-32% protein (biuret); 30% in saline
• free fatty acid content: ≤0.01%
• Technique(s): ELISA: suitable; electrophoresis: suitable; western blot: suitable
• Impuretés: ≤0.01% free fatty acid
• Numéro d'accès UniProt: P02769
• Activité étrangère: BT virus, none detected; VSV virus, none detected
• Température de stockage: 2-8°C
• Informations sur le gène: bovine ... ALB(280717)

Description

Description générale

Bovine Serum Albumin (BSA) has three homologous domains, each subdivided it two domain (A and B). It is a α-helical globular protein and shares structural features with human serum albumin.

Application

Bovine Serum Albumin solution has been used:

• as Dulbecco′s modified Eagle′s medium supplement for culturing HepG2 human liver cancer cells
• as a component of hepatocyte recovery medium
• as a component of fusion and activation buffers for in vitro reconstructed sheep embryos

Actions biochimiques/physiologiques

Bovine Serum Albumin (BSA) is the major protein present in blood and has calcium binding sites and regulates metal homeostasis. It is a crucial component of the cell culture medium and promotes embryonic stem cells (hESC) differentiation. Due to its high solubility, low cost and purity it is widely used in cosmetic and pharmaceutical industry. BSA is used as a blocking agent used in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates.

Notes préparatoires

Initial fractionation by cold alcohol precipitation

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)