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Principaux documents

A8887

Sigma-Aldrich

Acrylamide

suitable for electrophoresis, ≥99%

Synonyme(s) :

2-Propenamide, Polyacrylamide, acrylic amide, akrylamid, Acrylic acid amide

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About This Item

Formule linéaire :
CH2=CHCONH2
Numéro CAS:
Poids moléculaire :
71.08
Beilstein:
605349
Numéro CE :
Numéro MDL:
Code UNSPSC :
41105319
eCl@ss :
39031205
ID de substance PubChem :
Nomenclature NACRES :
NB.22
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Densité de vapeur

2.45 (vs air)

Niveau de qualité

Pression de vapeur

0.03 mmHg ( 40 °C)

Essai

≥99%

Forme

crystals

Technique(s)

electrophoresis: suitable

pb

125 °C/25 mmHg (lit.)

Pf

82-86 °C (lit.)

Solubilité

water: soluble 2.5 g/10 mL, clear, colorless

Adéquation

suitable for electrophoresis

Chaîne SMILES 

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

Clé InChI

HRPVXLWXLXDGHG-UHFFFAOYSA-N

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Description générale

Acrylamide acts as a quencher of tryptophan fluorescence.[1] Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis.[1] Bis-acrylamide is required for crosslinking and acrylamide is vital for the polymerization of monomers. Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels, which is used to separate protein and other biomolecules.[2]
Acrylamide (AA) is a neurotoxin and carcinogen. Potato products like, French fries, potato chips and also several cereal-based products contains acrylamide.[3] This highly reactive organic compound has the ability to polymerize to form polyacrylamide.[4]

Application

Acrylamide has been used:
  • for the synthesis of hydrogels[5][6]
  • for the preparation of polyacrylamide gel[7][8]
  • to agglomerate vimentin and keratin intermediate filament network[9]

Acrylamide has been used:
  • to agglomerate the vimentin and keratin intermediate filament network of K562cells[9]
  • in the preparation and characterization of soft culture substrate[8]
  • in hydrogel synthesis[10]
  • for determination of protein by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis[11]

Pictogrammes

Skull and crossbonesHealth hazard

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

Organes cibles

Peripheral nervous system

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

280.4 °F - closed cup

Point d'éclair (°C)

138 °C - closed cup

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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Certificats d'analyse (COA)

Lot/Batch Number

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Use of potato flour in bread and flat bread
Flour and Breads and Their Fortification in Health and Disease Prevention, 247-259 (2011)
Expression and Ni-NTA-Agarose Purification of Recombinant Hepatitis C Virus E2 Ectodomain Produced in a Baculovirus Expression System
Gomez-Gutirrrez J, et al.
Biochimica et Biophysica Acta - Biomembranes (2018)
A large deformation viscoelastic model for double-network hydrogels
Mao Y, et al.
Journal of the Mechanics and Physics of Solids, 100, 103-130 (2017)
Matrix stiffness modulates formation and activity of neuronal networks of controlled architectures
Lantoine J, et al.
Biomaterials, 89, 14-24 (2016)
Voltage-induced wrinkle performance in a hydrogel by dielectric elastomer actuation
Tang C, et al.
Polymer, 10(7), 697-697 (2018)

Questions

1–2 of 2 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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