A8580

Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit

IgG fraction of antiserum, PBS suspension

• Synonyme(s) : Anti-Glutathione-S-Transferase, Anti-GST
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.46

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Conjugué: agarose conjugate
• Forme d'anticorps: IgG fraction of antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: PBS suspension
• Poids mol.: antigen 27.5 kDa
• Ne doit pas réagir avec: porcine, bovine, human, rabbit, rat
• Technique(s): ELISA: suitable; immunoprecipitation (IP): suitable
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... GSTP1(2950)

Description

Description générale

Glutathione-S-transferases (GSTs) are dimeric proteins, which belongs to phase II detoxification enzymes family. The highly polymorphic human cytosolic GSTs are divided into six classes, such as, α, μ, ω, π, θ, and ζ.

The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.

Immunogène

recombinant GST from Schistosoma japonicum expressed in E. coli.

Application

Immunoprecipitation was performed on NIH 3T3 cells transiently transfected with GST fusion proteins. IP was performed using agarose-linked rabbit anti-GST IgG with mixing for 2 hours at 4 degrees.

Rabbit Anti-Glutathione-S-Transferase (GST)-Agarose antibody can be used for ELISA, immunoaffinity purification and immunoprecipitation assays.

Actions biochimiques/physiologiques

Glutathione-S-transferases (GSTs) can catalyse the conjugation of glutathione (GSH) to various endogenous and exogenous electrophilic compounds. The π and μ classes of GSTs controls the mitogen-activated protein (MAP) kinase pathway. It can also serve as transport proteins.

Forme physique

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Notes préparatoires

Prepared by coupling cyanogen bromide-activated agarose at 7-8 mg antibody per mL resin volume.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable