A7189

L-Alanine Dehydrogenase from Bacillus subtilis

ammonium sulfate suspension, ≥20 units/mg protein (Lowry)

• Synonyme(s) : L-Alanine: NAD⁺ oxidoreductase (deaminating)
• Numéro CAS: 9029-06-5
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-847-9
• MDL number: MFCD00131441
• Numéro CE : 1.4.1.1 (BRENDA, IUBMB)
• Specific activity: ≥20 units/mg protein (Lowry)
• Biological source: Bacillus subtilis

Propriétés

• biological source: Bacillus subtilis
• Quality Segment: 200
• form: ammonium sulfate suspension
• specific activity: ≥20 units/mg protein (Lowry)
• storage temp.: 2-8°C

Description

General description

L-Alanine Dehydrogenase has a N-terminal substrate-binding domain and a C-terminal NAD-binding domain.

Application

L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection .

L-Alanine Dehydrogenase from Bacillus subtilis has been used in the carbon nanotube columns for H2-driven biocatalysis hydrogenation studies.

Biochem/physiol Actions

L-Alanine dehydrogenase is a stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate and NADH are released in that specific order. Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.

L-Alanine Dehydrogenase is essential for sporulation in Bacillus subtilis.

Physical form

Suspension in 2.4 M (NH₄)₂SO₄ solution, pH 7.0

Other Notes

One unit will convert 1.0 μmole of L-alanine to pyruvate and NH₃ per min at pH 10.0 at 25 °C.

Informations sur la sécurité

• Classe de stockage: 12 - Non Combustible Liquids
• EPI (équipement de protection individuelle): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)