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A6782

Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in sheep

Name: Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in sheep
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

SAM

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A propos de cet article

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162644

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

ELISA (d)

Citations:

Service technique

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Propriétés

biological source

sheep

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3′,5,5′-tetramethylbenzidine (TMB), 3,3′-Diaminobenzidine (DAB) or 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS); chemiluminescent substrates such as CPS-3 (enhanced luminal) and fluorogenic substrates such as Ampliflu^™ Red into detectable chromophores, light-emitters or fluorescers, respectively.

Application

Sheep polyclonal anti-Mouse IgG (whole molecule)–Peroxidase antibody may be used to detect and quantitate the level of IgG in mouse serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques. It may also be used as a secondary antibody in assays that use mouse IgG as the primary antibody. Primary T cells isolated from buffy goats were analyzed by western blot using HRP-conjugated sheep anti-mouse IgG as the secondary antibody.

Biochem/physiol Actions

Sheep polyclonal anti-Mouse IgG (whole molecule)–Peroxidase antibody reacts with mouse IgG in vitro and in mouse serum and biological fluids. It does not react with human serum proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Other Notes

Antibody adsorbed with human serum proteins.

Legal Information

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.