A6255
α-Amylase from porcine pancreas
PMSF Treated, Type I-A, saline suspension, ≥1000 units/mg protein (E1%/280)
Synonyme(s) :
β-N-acetylglucosaminidase porcine placenta, PPA, al1,4 glucan-4-glucanohydrolase,, porcine pancreas α-amylase
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About This Item
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352204
eCl@ss :
32160410
Nomenclature NACRES :
NA.54
Produits recommandés
Source biologique
Porcine pancreas
Niveau de qualité
Type
Type I-A
Forme
saline suspension
Activité spécifique
≥1000 units/mg protein (E1%/280)
Poids mol.
51-54 kDa
Caractéristiques du produit alternatif plus écologique
Waste Prevention
Design for Energy Efficiency
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sustainability
Greener Alternative Product
Autre catégorie plus écologique
, Enabling
Conditions d'expédition
wet ice
Température de stockage
2-8°C
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Description générale
Molecular mass: 51-54 kDa.
α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7.
α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.
Application
α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. Product A6255 is from porcine pancreas and is type I-A. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis .
Actions biochimiques/physiologiques
α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. α -Amylase, from porcine pancreas, is a glycoprotein that consists of a single polypeptide chain of approximately 475 residues containing 2 SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability.
Définition de l'unité
One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.
Forme physique
Suspension in 2.9 M NaCl solution containing 3 mM CaCl2
Notes préparatoires
PMSF treated. 2× crystallized
Autres remarques
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Inhibiteur
Réf. du produit
Description
Tarif
Substrat
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificats d'analyse (COA)
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