A3010

Acylase I from porcine kidney

Grade I, lyophilized powder, ≥1500 units/mg protein

• Synonyme(s) : Aminoacylase, N-Acylamino acid amidohydrolase
• Numéro CAS: 9012-37-7
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-732-3
• MDL number: MFCD00081285
• Numéro CE : 3.5.1.14 (BRENDA, IUBMB)
• Specific activity: ≥1500 units/mg protein

Propriétés

• type: Grade I
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥1500 units/mg protein
• composition: Protein, ≥60%
• UniProt accession no.: P37111
• storage temp.: −20°C
• Gene Information: pig ... ACY1(396930)

Description

Application

Acylase I from porcine kidney has been used to study the acylase I-catalyzed deacetylation of various S-alkyl-N-acetyl-L-cysteines and their carbon and oxygen analogues . Acylase I may be useful to catalyze N-acetyl amino acids to enantiomerically pure L-amino acids .

Biochem/physiol Actions

Acylase I catalyzes the deacetylation of N-acetyl-L-cysteine and S-alkyl-N-acetyl-L-cysteines. n-Butylmalonic acid is an inhibitor of acylase I. S-alkyl-N-acetyl-L-cysteines with short (C0-C3) and unbranched S-alkyl substituents have been found to be good acylase I substrates .

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 μmole of N-acetyl-L-methionine per hr at pH 7.0 at 25 °C.

Informations sur la sécurité

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)