A276

Monoclonal Anti-Na⁺/K⁺ ATPase (α Subunit) antibody produced in mouse

clone M7-PB-E9, ascites fluid

• Synonyme(s) : Anti-CMT2DD, Anti-HOMGSMR2
• Numéro MDL: MFCD02096998
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: M7-PB-E9, monoclonal
• Poids mol.: antigen ~110 kDa
• Espèces réactives: canine, chicken, human, sheep, pig, bovine, mouse
• Ne doit pas réagir avec: rat, Xenopus
• Technique(s): immunocytochemistry: suitable; immunofluorescence: 1:20; immunohistochemistry (frozen sections): 1:100; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 1:500-1:5000
• Isotype: IgG1
• Numéro d'accès UniProt: P05023
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ATP1A1(476)

Description

Description générale

The Na⁺/K⁺ ATPase (NKA) enzyme contains α, β and γ subunits. This enzyme is widely expressed. ATPase Na⁺/K⁺ transporting subunit α 1 (ATP1A1) is a principal catalytic subunit of NKA. It has a molecular mass of about 110kDa with 10 transmembrane segments. α-subunit has four different isoforms with tissue specific expression. α1 is primarily expressed in various tissues, including kidney, nerves and lung. While α2 is expressed in skeletal muscle and heart, α3 is found in the brain and α4 is apparently localized to testis and specifically to spermatozoa. ATP1A1 is encoded by the gene mapped to human chromosome 1p13-p11.

Immunogène

sheep kidney α subunit of Na⁺/K⁺ ATPase.

Application

Monoclonal Anti- Na⁺/K⁺ ATPase ( α Subunit) antibody produced in mouse has been used in flow cytometry.

Actions biochimiques/physiologiques

The Na⁺/K⁺ ATPase is implicated in the maintenance of ion homeostasis for regular neuronal excitability, secondary transport and neurotransmitter uptake. ATPase Na⁺/K⁺ transporting subunit α 1 (ATP1A1) participates in both pumping and signaling functions. ATP1A1 regulates cellular transmembrane sodium and potassium ion gradients essential for the control of resting membrane potential. Additionally, nucleotide binding domain of ATP1A1 facilitates cellular signal transduction and cell growth. Deficiency of the gene leads to development of major depressive disorder (MDD).

The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the α subunit, and an associated smaller glycoprotein, termed the β subunit. Studies indicate that there are three isoforms of the α subunit (α 1, α 2, α 3) and two isoforms of the β subunit (β 1 and β 2) encoded by two multigene families.
The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The α 1 and β mRNAs are present in all cell types examined, whereas the α 2 and α 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The α subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.

Description de la cible

Na⁺/K⁺ transporting ATPase subunit α1 is an ion transport pump critical for maintaining the gradient of Na and K ions across the plasma membrane.

Forme physique

solution containing 1 mg/ml BSA and 0.05% sodium azide

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)