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94742

Sigma-Aldrich

Vanadyl ribonucleoside complexes solution

BioReagent, for molecular biology, 200 mM

Synonyme(s) :

Ribonucleoside vanadyl complexes

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.52

Qualité

for molecular biology

Niveau de qualité

Gamme de produits

BioReagent

Forme

liquid

Concentration

200 mM

Impuretés

oxovanadium V

Adéquation

in accordance for ribonuclease inhibition test

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Vanadyl ribonucleoside complexes are low molecular weight inhibitors of ribonucleases.
Vanadyl ribonucleoside complexes solution is an inhibitor for ribonuclease (RNase). Use of vanadyl ribonucleoside complexes exhibits protection from degradation by RNase in tissue, genomic, RNA and protein samples.

Application

Vanadyl ribonucleoside complexes (VDR) are transition state analogs that bind to active sites of many RNases, inhibiting their activity. Because the RNases are not covalently modified by the complexes, VDR must be used at every stage of RNA extraction and purification. However, VDR also inhibit RNA polymerases and in vitro translation and therefore must be removed from final preparation of RNA.
Vanadyl ribonucleoside complexes solution has been used:
  • as a component of polysome lysis buffer and fluorescence in situ hybridization (FISH) buffer
  • as a RNase inhibitor for the isolation of endogenous YTH domain-containing 2 protein (YTHDC2) from mouse testes

Produit(s) apparenté(s)

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Wei Wang et al.
Nucleic acids research, 36(15), 4913-4928 (2008-07-26)
The mechanisms of influenza A virus mRNA intracellular transport are still not clearly understood. Here, we visualized the distribution and transport of influenza A virus mRNA in living cells using molecular beacon (MB) technology. Confocal-FRAP measurements determined that the transport
CARM1 and Paraspeckles Regulate Pre-implantation Mouse Embryo Development
Hupalowska A, et al.
Cell, 175(7), 1902-1916 (2018)
Immunoprecipitation of mRNA-protein complexes
Peritz T, et al.
Nature Protocols, 1(2), 577-577 (2006)
Single-molecule Fluorescence in situ Hybridization (smFISH) for RNA Detection in Adherent Animal Cells
Haimovich G and Gerst JE
Proceedings of the National Academy of Sciences of the USA, 8(21) (2018)
Regulation of m 6 A Transcripts by the 3?? 5? RNA Helicase YTHDC2 Is Essential for a Successful Meiotic Program in the Mammalian Germline
Wojtas MN, et al.
Molecular Cell, 68(2), 374-387 (2017)

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