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Sigma-Aldrich

Phalloidin–Atto 565

suitable for fluorescence, ≥80.0% (HPLC)

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About This Item

Code UNSPSC :
12352108
Nomenclature NACRES :
NA.32

Niveau de qualité

100

Pureté

≥80.0% (HPLC)

Forme

solid

Poids mol.

Mw 1394 g/mol

Fabricant/nom de marque

ATTO-TEC GmbH

λ

in methanol

Absorption UV

λ: 562.0-568.0 nm Amax

Adéquation

suitable for fluorescence

Température de stockage

−20°C

Description générale

Atto 565 is a novel fluorescent label that belongs to the class of Rhodamine dyes. It shows a strong absorption, high fluorescence quantum yield, high thermal and photostability, and a very little triplet formation. Atto 565 consists of a mixture of two isomers with practically identical optical absorption and emission Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10-8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours. Fluorescent conjugates of phalloidin are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-d-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Atto 565 BioReagent, suitable for fluorescence

Sigma-Aldrich

75784

Atto 565

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