67060

Methyl Green

zinc chloride salt, for microscopy (Bact., Bot., Hist.)

• Synonyme(s) : Ethyl Green
• Formule linéaire : C₂₇H₃₅BrClN₃ · ZnCl₂
• Numéro CAS: 7114-03-6
• Poids moléculaire : 653.24
• Numéro C.I. (Colour Index): 42590
• Numéro Beilstein : 3914106
• Numéro CE : 230-415-4
• Numéro MDL: MFCD00151094
• Code UNSPSC : 12171500
• ID de substance PubChem : 329760277
• Nomenclature NACRES : NA.47

Propriétés

• Qualité: for microscopy (Bact., Bot., Hist.)
• Forme: powder
• pH: 0.2-1.8, yellow to blue
• Pf: >300 °C (lit.)
• Solubilité: water: 0.01 g/10 mL, blue to very deep blue
• εmax: ≥900 at 624-634 nm in water
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: [Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C
• InChI: 1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4
• Clé InChI: RRKSQWOQZFIQKW-UHFFFAOYSA-J

Description

Application

Methyl green has been used in the histological staining of ovaries. It has also been used as a counterstain in various cells.

Actions biochimiques/physiologiques

Marker dye for DNA in acid and neutral media.
Methyl green is mainly used as a DNA stain in histochemistry. At low concentrations it binds selectively to AT-rich regions of native DNA. However, at high concentrations it is present at all available DNA sites.

Remarque sur l'analyse

Material may be a mixture of monobromo-monochloro and dichloro ZnCl₂ salts.

Informations sur la sécurité

• Pictogrammes: GHS05,GHS07,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H314,H335,H411
• Conseils de prudence: P260 - P271 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Classification des risques: Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 8A - Combustible corrosive hazardous materials
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves