46058
Esterase from porcine liver
lyophilized, powder, slightly beige, ≥50 U/mg
Synonyme(s) :
Carboxyl esterase, Carboxylic-ester hydrolase, PLE
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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-773-7
MDL number:
Specific activity:
≥50 U/mg
Biological source:
Porcine liver
Service technique
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Porcine liver
Quality Segment
form
lyophilized solid, powder
quality
lyophilized
specific activity
≥50 U/mg
mol wt
Mr ~162000
color
slightly beige
storage temp.
−20°C
General description
Porcine liver esterase (PLE) is localized in the endoplasmic reticulum (ER).
Application
Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.
Esterase from porcine liver has been used as a negative control in fluorescence measurement studies.
Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications.
Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.
Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.
Biochem/physiol Actions
Esterase acts on water-soluble carboxyl esters containing short chain fatty acids. Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu.
Porcine liver esterase (PLE) displays good stability, broad substrate specificity, and is a low-cost enzyme. It is useful in the hydrolysis of ester- and amide-containing compounds, to free acids and is useful detoxification of xenobiotics.
Other Notes
1 U correspond à la quantité d′enzyme qui hydrolyse 1 μmol d′éthyl valérate (Fluka® No. 30784) par minute à pH 8.0 et 25°C
Contient du 1,4-dithioérythritol
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Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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