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18507

Sigma-Aldrich

Atto 565 maleimide

BioReagent, suitable for fluorescence

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About This Item

Formule empirique (notation de Hill):
C37H37ClN4O10
Poids moléculaire :
733.16
Numéro MDL:
MFCD04040675
Code UNSPSC :
12352111
Nomenclature NACRES :
NA.32

Gamme de produits

BioReagent

Niveau de qualité

200

Pureté

>90.0% (HPLC)

Forme

powder

Fabricant/nom de marque

ATTO-TEC GmbH

Transmittance

254 nm
565 nm

Fluorescence

λex 563 nm; λem 592 nm in 0.1 M phosphate pH 7.0

λ

(ethanol with 0.1% trifluoroacetic acid)

Absorption UV

λ: 560-566 nm Amax

Adéquation

corresponds for coupling to thiols
suitable for fluorescence

Température de stockage

−20°C

Application

Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

Informations légales

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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