11214667001
Roche
Anti-Digoxigenin, Fab fragments
from sheep
Synonyme(s) :
anti-digoxigenin, digoxigenin
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About This Item
Code UNSPSC :
12352203
Produits recommandés
Source biologique
sheep
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
affinity purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
lyophilized
Conditionnement
pkg of 1 mg
Fabricant/nom de marque
Roche
Technique(s)
ELISA: 2-4 μg/mL
immunohistochemistry: 0.5-1 μg/mL
western blot: 0.5-2 μg/mL
Isotype
IgG1
Température de stockage
2-8°C
Description générale
Anti-Digoxigenin, Fab fragments are Fab fragments from polyclonal anti-digoxigenin antibodies. Anti-Digoxigenin Fab fragments are preferentially used for secondary detection using a light chain specific anti-sheep conjugate.
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems.
Spécificité
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
Application
Anti-Digoxigenin Fab fragments are preferentially used for secondary detection using a light chain specific anti-sheep conjugate.
Use Anti-Digoxigenin, Fab fragments for the detection of digoxigenin-labeled compounds using:
Use Anti-Digoxigenin, Fab fragments for the detection of digoxigenin-labeled compounds using:
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Western blot
- Flow cell construction.
Caractéristiques et avantages
Specific to digoxigenin and digoxin.
Séquence
The number of amino groups in the Fab fragments is not known, because polyclonal antibodies are not sequenced. But as a rule of thumb full length antibodies contain about 80 amino groups on average.
Notes préparatoires
Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
Storage conditions (working solution): Diluted antibody is stable at 2 to 8 °C for 12 hours.
Always prepare freshly!
- ELISA: 2 to 4 μg/ml
- Immunohistocytochemistry: 0.5 to 1 μg/ml
- In situ hybridization: 0.2 to 0.4 μg/ml
- Western blot: 0.5 to 2 μg/ml
Storage conditions (working solution): Diluted antibody is stable at 2 to 8 °C for 12 hours.
Always prepare freshly!
Reconstitution
Add 1 ml PBS to a final concentration of 1 mg/ml. Centrifuge reconstituted antibody for 5 minutes at 10,000 rpm prior to each use, and pipet the necessary amount carefully from the surface.
Remarque sur l'analyse
Preparation: After immunization with digoxigenin, sheep IgGs are purified by ion exchange chromatography. Specific IgGs are isolated by immunosorption. Fab fragments obtained after papain digestion are purified by gel filtration and lyophilized.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
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Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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