06432379001
Roche
Cell Viability Imaging Kit 5x96
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.55
Produits recommandés
Utilisation
sufficient for 5 96-well plate(s)
Niveau de qualité
Conditionnement
pkg of 1 kit
Fabricant/nom de marque
Roche
Conditions de stockage
protect from light
Fluorescence
λex 361 nm; λem 486 nm (nuclei dye)
λex 490 nm; λem 515 nm (viable dye)
λex 535 nm; λem 617 nm (dead dye)
Méthode de détection
fluorometric
Conditions d'expédition
dry ice
Température de stockage
−20°C (−15°C to −25°C)
Description générale
The Cell Viability Imaging Kit is a simple and rapid way to measure mammalian cell viability using fluorescence microscopy and automated imaging platforms, such as the Cellavista System. Optimized for 96-well plates, the numbers of viable and dead cells, as well as total cell numbers, are determined simultaneously for the area of the well selected.
The kit is designed for 5 × 96 reactions for use in medium to high throughput analysis of cell behavior.
- Determine total cell number using fluorescence staining of cell nuclei.
- Fluorimetrically determine the percentage of dead cells based on membrane permeability.
- Verify the labeling of viable cells quantified by fluorescence staining using a vital dye
Application
Cell quantification and cell viability control are very important methods in cell biology. This includes both life science research and industrial applications. The simple, accurate and fast “one step assay” makes the Cell Viability Imaging Kit an ideal tool for quality control of cells in cellular workflows, especially for medium to high throughput applications.
Caractéristiques et avantages
Cell counting and cell viability are essential for cellular workflows. The Roche Cell Viability Imaging Kit meets the following requirements:
- No risk of losing cells in the one step assay: There are no fixation or washing steps.
- Save time and resources: Total assay time for a 1 x 96 well microplate is only 35 minutes.
- Obtain highly reproducible results using automated imaging and data evaluation with Roche′s Cellavista System.
Conditionnement
1 kit containing 3 components.
Notes préparatoires
Storage conditions (working solution): Vial 3: The product is stable until the expiration date printed on the label when stored at -15 to -25 °C. Contents can be frozen and thawed at least 5 times after preparation of solution.
Stockage et stabilité
Store at -15–-25 °C. (Vial 1 and Vial 2 can be frozen and thawed at least 5 times. Vial 3 can be frozen and thawed at least 5 times after preparation of solution.)
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Composants de kit seuls
Réf. du produit
Description
- Nuclei Dye (blue cap) for fluorimetric determination of cell count based on labeling of cell nuclei
- Dead Dye (red cap) for fluorimetric determination of dead cells based on cell permeability
- Viable Dye ( green cap) for fluorimetric determination of viable cells based on metabolic activity
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Muta. 2
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Fengliang Wang et al.
Molecular medicine reports, 16(4), 5441-5449 (2017-08-30)
Previous studies have reported that angelicin exerted antiproliferative effects on several types of tumor cell. However, to the best of our knowledge, the effects of angelicin monotherapy on human liver cancer remain to be investigated. In the present study, the
Lingbo Xu et al.
Molecular medicine reports, 23(6) (2021-04-22)
Our previous study reported that microRNA (miR)‑30a‑5p upregulation under hypoxia postconditioning (HPostC) exert a protective effect on aged H9C2 cells against hypoxia/reoxygenation injury via DNA methyltransferase 3B‑induced DNA hypomethylation at the miR‑30a‑5p gene promoter. This suggests that miR‑30a‑5p may be
Kun Xiao et al.
Gut pathogens, 13(1), 63-63 (2021-10-21)
The liver plays an important role in production and metabolism of homocysteine (Hcy), which has been reported to be involved in liver injury. In our previous work, we confirm that Hcy can induce liver injury by activating endoplasmic reticulum (ER)
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique